Journal: Clinical and Translational Science

Article Title: Gut Microbiota Composition Correlates to Trunk Fat Percentage in Male Cynomolgus Macaque Monkeys

doi: 10.1111/cts.70393

Figure Lengend Snippet: Insulin and triglyceride parameters were associated with trunk fat percentage. Twenty‐three male cynomolgus monkeys (aged 5–6 years) were fed high fat diet for 4.5 years and their metabolic state was evaluated according to trunk fat percentage. The trunk fat percentage were divided into quartiles: 1: Minimun‐25th percentile ( n = 6) ‘slightly overweight’, 2: 25th–50th percentile ( n = 6) ‘overweight’, 3: 50th—75th percentile ( n = 6) ‘obese’, 4: 75th percentile—maximum ( n = 5) ‘very obese’. (A) Trunk fat percentage (TF%) of the quartiles; Fasting plasma concentration of (B) total triglycerides (mg/dL), (C) total cholesterol (mg/dL), (D) LDL‐ and HDL‐cholesterol (mg/dL), (E) glucose (mg/dL) and (F) insulin (μU/mL); (G) HbA1c (%), (H) insulin sensitivity index and (I) Homeostatic model assessment for insulin resistance (HOMA‐IR). Data are shown as individual values with mean ± SD. One‐way ANOVA adjusting for multiple comparison using the FDR method of Benjamini, Hochberg, and Yekutieli was used. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001 mark the level of statistical significance.

Article Snippet: Pearson correlation matrix was used to evaluate associations between variables (log transformed data), while adjusting for the number of correlations using FDR (R software v4.1.1 and package psych v2.1.9; Minitab v22.2.1. (Coventry, UK), PRISM).

Techniques: Clinical Proteomics, Concentration Assay, Comparison

Journal: Clinical and Translational Science

Article Title: Gut Microbiota Composition Correlates to Trunk Fat Percentage in Male Cynomolgus Macaque Monkeys

doi: 10.1111/cts.70393

Figure Lengend Snippet: The ratio of glycine‐to‐taurine‐conjugated cholic acid correlated negatively to trunk fat percentage and HOMA‐IR. Twenty‐three male cynomolgus monkeys (aged 5–6 years) were fed high fat diet for 4.5 years and had the bile acid pool assessed in a fasted state. Circulating bile acids (BA) species were measured and visualized in (A) Stacked barchart of bile acid (BA) species (ng/mL) and (B) The ratio of glycine‐ to taurine‐ conjugated BA. Data are shown as individual values with mean ± SD. One‐way ANOVA adjusting for multiple comparison using FDR method of Benjamini, Hochberg, and Yekutieli was used. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001 mark the level of statistical significance. The trunk fat percentage were divided into quartiles: 1: Minimun‐25th percentile ( n = 6) ‘slightly overweight’, 2: 25th–50th percentile ( n = 6) ‘overweight’, 3: 50th—75th percentile ( n = 6) ‘obese’, 4: 75th percentile—maximum ( n = 5) ‘very obese’; (C–E) Pearson correlation matrix using log10 transformed data, yellow circles indicate positive correlations and green circles indicate negative correlations. FDR adjusted q values are added as number and written in bold if significant and italics if borderline significant.

Article Snippet: Pearson correlation matrix was used to evaluate associations between variables (log transformed data), while adjusting for the number of correlations using FDR (R software v4.1.1 and package psych v2.1.9; Minitab v22.2.1. (Coventry, UK), PRISM).

Techniques: Comparison, Transformation Assay